LOCUS       sequence-140857-        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of N-terminal fusion of mClover3 to Tubulin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pKanCMV-mClover3-18aa-Tubulin
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(28..30,31..33,34..744)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(73..94)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     681..702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgtctaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
      121 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc
      241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccactacgtc
      481 tatatcacgg ccgacaagca gaagaactgc atcaaggcta acttcaagat ccgccacaac
      541 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggattaca
      721 catggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg aggcagcgca
      781 tccggcggaa gcggaagcgt gcgtgagtgc atctccatcc acgttggcca ggctggtgtc
      841 cagattggca atgcctgctg ggagctctac tgcctggaac acggcatcca gcccgatggc
      901 cagatgccaa gtgacaagac cattggggga ggagatgact ccttcaacac cttcttcagt
      961 gagacgggcg ctggcaagca cgtgccccgg gctgtgtttg tagacttgga acccacagtc
     1021 attgatgaag ttcgcactgg cacctaccgc cagctcttcc accctgagca gctcatcaca
     1081 ggcaaggaag atgctgccaa taactatgcc cgagggcact acaccattgg caaggagatc
     1141 attgaccttg tgttggaccg aattcgcaag ctggctgacc agtgcaccgg tcttcagggc
     1201 ttcttggttt tccacagctt tggtggggga actggttctg ggttcacctc cctgctcatg
     1261 gaacgtctct cagttgatta tggcaagaag tccaagctgg agttctccat ttacccagca
     1321 ccccaggttt ccacagctgt agttgagccc tacaactcca tcctcaccac ccacaccacc
     1381 ctggagcact ctgattgtgc cttcatggta gacaatgagg ccatctatga catctgtcgt
     1441 agaaacctcg atatcgagcg cccaacctac actaacctta accgccttat tagccagatt
     1501 gtgtcctcca tcactgcttc cctgagattt gatggagccc tgaatgttga cctgacagaa
     1561 ttccagacca acctggtgcc ctacccccgc atccacttcc ctctggccac atatgcccct
     1621 gtcatctctg ctgagaaagc ctaccatgaa cagctttctg tagcagagat caccaatgct
     1681 tgctttgagc cagccaacca gatggtgaaa tgtgaccctc gccatggtaa atacatggct
     1741 tgctgcctgt tgtaccgtgg tgacgtggtt cccaaagatg tcaatgctgc cattgccacc
     1801 atcaaaacca agcgcagcat ccagtttgtg gattggtgcc ccactggctt caaggttggc
     1861 atcaactacc agcctcccac tgtggtgcct ggtggagacc tggccaaggt acagagagct
     1921 gtgtgcatgc tgagcaacac cacagccatt gctgaggcct gggctcgcct ggaccacaag
     1981 tttgacctga tgtatgccaa gcgtgccttt gttcactggt acgtgggtga ggggatggag
     2041 gaaggcgagt tttcagaggc ccgtgaagat atggctgccc ttgagaagga ttatgaggag
     2101 gttggtgtgg attctgttga aggagagggt gaggaagaag gagaggaata ctaaggatcc
     2161 accggatcta gataactgat cataatcagc cataccacat t
//
