LOCUS       sequence-140859-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector bearing an NheI restriction site for the two-step cloning 
            method in Wang and Szaro, Dev Biol, 2015.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tcaagcttgg
       61 ctagattttg aatgggtcat ttattggatc ttcctccctg atttttcagt gtacctagca
      121 gcaatcccag gcaatgttat tatcactcac tttgtctcta actcatcatc tctataaatt
      181 ttcccatgta ctattctctg ggcatgctgg caagaactga tctagatctc caagttaacc
      241 atgtcatcag gattggctaa aatttgtaag catctgtgca taaatctgtg tattctattc
      301 ctattttgag catatatttt ctttcatgga ttcagccatg cttttagcca gggtatatga
      361 tctctgtgtc accagctata ttagatagcc ctcaggctca gttgctccta ggtaatgagg
      421 attagagtgt tttagtgagc agggggttaa gttgctactt gagacaccaa catcccatat
      481 caaagtgttt attctggtcc ccacagctcc acttctgatc tagttttctg ctaatgtacc
      541 tggaagcagc cgaatatggc tcatgtactt gagtccctgc cacccatcat ggagacccca
      601 tggcaattcc tgttttctgg ttttggcctg acacactcct gactgttgca ggtatttggg
      661 gagtgaacca gaacctttca tccacctgag tgcctattga atgaggaaat ggtgacagta
      721 agctaatcac tacagagaca gcaaaaccat cagacacagg ataaaggagt gggcaactct
      781 gcaatcactt tctgaattca gaagctaaca tcctgaggga ctgtcatttg atttgagtcc
      841 ataaaaaggg tttaagacaa cagtgaatat aaagctgcac aaagccccct tttgcatcca
      901 gtttattcct acatttgtca cactgttaac agcccacccc ttccaatgag accagtggta
      961 tcagtgagtt gtggagatca ggaaaagggc tcaagagaaa ggcagtcaaa gcccttttt
//
