LOCUS       sequence-140865-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of N-terminal fusion of mRuby3 to actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pKanCMV-mRuby3-18aa-actin
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..750
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgtctaa gggcgaagag
       61 ctgatcaagg aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa
      121 ttcaaatgca caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc
      181 aaagtcatcg agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcatg
      241 tatggcagcc gtacctttat caagtacccg gccgacatcc ctgatttctt taaacagtcc
      301 tttcctgagg gttttacttg ggaaagagtt acgagatacg aagatggtgg agtcgtcacc
      361 gtcacgcagg acaccagcct tgaggatggc gagctcgtct acaacgtcaa ggtcagaggg
      421 gtaaactttc cctccaatgg tcccgtgatg cagaagaaga ccaagggttg ggagcctaat
      481 acagagatga tgtatccagc agatggtggt ctgagaggat acactgacat cgcactgaaa
      541 gttgatggtg gtggccatct gcactgcaac ttcgtgacaa cttacaggtc aaaaaagacc
      601 gtcgggaaca tcaagatgcc cggtgtccat gccgttgatc accgcctgga aaggatcgag
      661 gagagtgaca atgaaaccta cgtagtgcaa agagaagtgg cagttgccaa atacagcaac
      721 cttggtggtg gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc
      781 agcgcatccg gcggaagcgg aagcgatgat gatatcgccg cgctcgtcgt cgacaacggc
      841 tccggcatgt gcaaggccgg cttcgcgggc gacgatgccc cccgggccgt cttcccctcc
      901 atcgtggggc gccccaggca ccagggcgtg atggtgggca tgggt
//
