LOCUS       sequence-140866-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Peredox fluorescent sensor protein (for NADH/NAD 
            ratio) in bacteria, with N-terminal his-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Mongeon R, Venkatachalam V, Yellen G
  TITLE     Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon 
            Fluorescence Lifetime Biosensor Imaging.
  JOURNAL   Antioxid Redox Signal. 2016 Mar 18.
  PUBMED    26857245
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antioxid 
            Redox Signal. 2016 Mar 18."
COMMENT     Sequence Label: pRSetB-Peredox
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgaaac atcaccatca tcatcatcat
       61 ggtggagcta gcatgaaagt tcctgaagca gccatttcca gactgattac ttacctgaga
      121 atcctggaag agctggaagc ccaaggtgtt caccgcaccg cctccgagca actgggcgag
      181 ctggcccagg tcaccgcctt ccaggttgat aaggacctgt cctacttcgg ctcctacggc
      241 actgacggcg ttggctacac tgtaccggtc ctcaagagag aactgcgcca tatcctcggt
      301 ctgaaccgca aatggggcct gtgcatcgtg gggatgggcc gcctgggatc cgctcttgct
      361 gattggcctg gttttggcga gagcttcgag ctgcgcggtt tcttcgatgt tgacccaggt
      421 atggttggtc ggccggttcg cggtggtgtt atcgaacacg ttgatctgct gccccagcgc
      481 gtacctggtc gcatcgaaat cgctctgctg acggttccac gcgaggcagc acagaaggct
      541 gccgacctgc tggttgccgc tggcatcaag ggcatcctga acttcgctcc agttgtactg
      601 gaggttccca aggaagtggc tgttgagaac gttgacatcc tggccggtct gacgcgtctg
      661 agctttgcca ttctgaaccc cacgtggagc gcagcaggtg ggcatggttt cactgctcac
      721 aacgtgtata tcatggctga caaacagaag aacggtatca aggccaactt caagatccgc
      781 cacaac
//
