LOCUS       sequence-140885-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded superfolder GFP in universal C-terminal tagging 
            plasmid, hygB resistanc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS286 C terminal SF-GFP hygBR
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg ctctagaggt
       61 gctggatctg gttcattaat taacatggtt tctaagggtg aagaactttt tactggtgtt
      121 gttcctattc ttgttgaact tgatggtgat gttaacggtc ataagttttc tgttcgtggt
      181 gaaggtgaag gtgatgctac taatggtaaa cttactctta agtttatttg cactactggt
      241 aagttgcctg ttccttggcc tactttagtt actactctta cttatggtgt tcaatgcttt
      301 tctcgttatc ctgatcatat gaagcaacat gattttttta agtctgctat gcctgaaggt
      361 tatgttcaag aacgtactat ttcttttaag gatgatggta cttacaagac tcgtgctgaa
      421 gttaagtttg aaggtgatac tcttgttaat cgtattgaac ttaagggtat tgattttaaa
      481 gaagatggta atattcttgg tcataagctt gaatacaact ttaattctca taacgtttac
      541 attactgctg ataagcaaaa gaacggtatt aaggctaact ttaagattcg tcataatgtt
      601 gaagatggtt ctgttcaact tgctgatcat taccaacaaa acactcctat tggtgatggt
      661 cctgttctcc ttcctgataa ccattacctt tctactcaat ctgttctttc taaggatcct
      721 aacgaaaagc gtgatcatat ggttcttttg gaatttgtta ctgctgctgg tattactctt
      781 ggtatggatg aactttacaa gtaaaaaaag actaatgtaa aatttttttg gttggttatt
      841 gaaaaagtcg atgccttgtt tgcgtttgtt tt
//
