LOCUS       sequence-140887-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            hygB resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS287 C terminal E2C hygBR
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaggtgctgg
       61 atctggttca ttaattaaca tggattctac tgaaaacgtt attaagcctt ttatgcgttt
      121 taaggttcat atggaaggtt ctgttaatgg tcatgaattt gaaattgaag gtgttggtga
      181 aggtaagcct tatgaaggta ctcaaactgc taagcttcaa gttacaaaag gtggtccttt
      241 accttttgct tgggatattc tttctcctca atttttttac ggttctaaag cttacattaa
      301 gcatcctgct gatattccag attaccttaa gcaatcattt cctgaaggtt ttaagtggga
      361 acgtgttatg aactttgaag atggtggtgt tgttactgtt actcaagatt cttcacttca
      421 agatggtact cttatttacc atgttaagtt tattggtgtt aattttcctt ctgatggtcc
      481 tgttatgcaa aaaaagactt taggttggga accttctaca gaacgtaatt atcctcgtga
      541 tggtgttctt aagggtgaaa atcatatggc tcttaagttg aaaggtggtg gtcattacct
      601 ttgcgaattt aagtctattt acatggctaa gaagcctgtt aagcttcctg gttatcatta
      661 cgttgattac aagcttgata ttacttctca taatgaagat tatactgttg ttgaacaata
      721 tgaacgtgct gaagctcgtc atcatctttt tcaataaaaa aagactaatg taaaattttt
      781 ttggttggtt attgaaaaag tcgatgcctt gtttgcgttt gttttcctag gcgttttatg
      841 tcagaaggca tttagaatta gtatacaagt actctttggt aaaattttat gtagcgacta
      901 aaatattaac tattatagat aaacaccttg ggaataaaaa gtaatttgct atagtaattt
      961 attaaacatg ctcctacaac attaccacaa tcttttctct tggattgaca ttgaataaga
     1021 aa
//
