LOCUS       sequence-140902-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV vector to express PBG (chimeric Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Kim EJ, Juavinett AL, Kyubwa EM, Jacobs MW, Callaway EM
  TITLE     Three Types of Cortical Layer 5 Neurons That Differ in Brain-wide 
            Connectivity and Function.
  JOURNAL   Neuron. 2015 Dec 16;88(6):1253-67. doi: 
            10.1016/j.neuron.2015.11.002. Epub 2015 Dec 6.
  PUBMED    26671462
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.11.002"; journalName: "Neuron"; date: 
            "2015-12-16- 16"; volume: "88"; issue: "6"; pages: "1253-67"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-PBG-WPRE-hGH
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..70)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(121..154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgtgaggta ccggatcctc tagagtcgac tccggaataa cttcgtatag gatactttat
       61 acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta
      121 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccttacagtc tggtctcacc
      181 cccactcttg tgtgattccc atgaagatat gatcttcccg ctttggggag tgactgacac
      241 ctccctccct gtccctctga gattgtgttg cgtaggttct gatcgattga ctcttctcca
      301 gcatgtcatc aggaaaatta tcaacatcaa ggcagtcagg gcccctgcac tcagtaatac
      361 atacttcccc cagttcggga gacccaagtc aactcctgag atccgttcgt gcacatcggg
      421 aaggtgaact tcaacaaaat cctcagcctc gtcaccgttc ttgaaaacgg tagacgggtc
      481 tgccaggggg tgcataaggg ggataaccga ggataccaac aactccatat gttgctggag
      541 gagggatgat tgcatctctg ggattaagac attgccgtca ggtcctaata ttataccatt
      601 gaaaaatacc ccgtttacat gaggatgaca cctcccccca actcttaaac acccttttga
      661 agggatgatc tcattccaag ttctgactga cttgtagtga gcatcggctt ccatcaaggt
      721 cttgttgaat atggtatatg cttttccaaa cccagggaca agttttctta aatgactgag
      781 acgtctgaaa ctcactgact tggtggtcat gatggactct agtgcatcca gacactcctc
      841 tctcttcttg accaactcct ctacaacaag gtgctcaatt tcgtctgagc gaaagtcgtg
      901 caa
//
