LOCUS       sequence-140905-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV vector to express PBG (chimeric Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kim EJ, Juavinett AL, Kyubwa EM, Jacobs MW, Callaway EM
  TITLE     Three Types of Cortical Layer 5 Neurons That Differ in Brain-wide 
            Connectivity and Function.
  JOURNAL   Neuron. 2015 Dec 16;88(6):1253-67. doi: 
            10.1016/j.neuron.2015.11.002. Epub 2015 Dec 6.
  PUBMED    26671462
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.11.002"; journalName: "Neuron"; date: 
            "2015-12-16- 16"; volume: "88"; issue: "6"; pages: "1253-67"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-PBG-WPRE-hGH
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag cgccgccacc
      181 atggttcctc aggctctcct gtttgtaccc cttctggttt ttccattgtg ttttgggaaa
      241 ttccctattt acacgatacc agacaagctt ggtccctgga gcccgattga catacatcac
      301 ctcagctgcc caaacaattt ggtagtggag gacgaaggat gcaccaacct gtcagggttc
      361 tcctacatgg aacttaaagt tggatacatc tcagccataa aaatgaacgg gttcacttgc
      421 acaggcgttg tgacggaggc tgaaacctac actaacttcg ttggttatgt cacaaccacg
      481 ttcaaaagaa agcatttccg cccaacacca gatgcatgta gagccgcgta caactggaag
      541 atggccggtg accccagata tgaagagtct ctacacaatc cgtaccctga ctaccactgg
      601 cttcgaactg taaaaaccac caaggagtct ctcgttatca tatctccaag tgtggcagat
      661 ttggacccat atgacagatc ccttcactcg ccggtcttcc ctggcgggaa ttgctcagga
      721 gtagcggtgt cttctaccta ctgctccact aaccacgatt acaccatttg gatgcccgag
      781 aatccgagac tagggatgtc ttgtgacatt tttaccaata gtagagggaa gagagcatcc
      841 aaagggagtg agacttgcgg ctttgtagat gaaagaggcc tatataagtc tttaaaa
//
