LOCUS       sequence-140915-         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD13A
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gccccgccgc ctacggtctc aagccgctga ccccgaacag caaatacgtg
      121 aagctgaacg tgggcggctc gttgcactac accacgctgc gcaccctcac gggacaggac
      181 accatgctca aagccatgtt cagcggccgc gtggaggtgc tgaccgatgc cggaggttgg
      241 gtgctgattg accggagcgg ccgtcacttt ggtacaatcc tcaattacct gcgggatggg
      301 tctgtgccac tgccggagag tacgagagaa ctgggggagc tgctgggcga agcacgctac
      361 tacctggtgc agggcctgat tgaggactgc cagctggcgc tgcagcaaaa aagggagacg
      421 ctgtgacagt aaaggtggat acggatccga a
//
