LOCUS       sequence-140917-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPIN3A
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             649..669
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             670..687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             688..711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcctcggg ggaacatcgt gggctgcaga attcagcacg gatggaaaga tggagatgaa
       61 cctctaacac agtggaaagg aaccgttctg gatcaggtac ctgtaaatcc ctctctgtat
      121 cttatcaaat atgatggatt tgactgtgtt tatggattag aacttcacag agatgaaaga
      181 gtgtcatcac ttgaagtcct tcctaataga gttgcatcat ctagaatcag tgatacacac
      241 ttggcagaaa taatggttgg caaggcagtg gaacatattt ttgagacaga ggaaggttcc
      301 aaaaatgaat ggagggggat ggtcttagct caggcacctg tcatgaacac atggttttac
      361 attacctatg agaaagatcc tgtattatat atgtaccagc tcttagatga ttataaagat
      421 ggtgacctcc gcatccttca agattccaat gattctcctc tggcagagag ggagccagga
      481 gaagtcatag acagcctggt aggcaaacag gtggaatacg ccaaagacga tggctccaag
      541 agaactggca tggtcattca tcaggtagaa gcaaaaccct ctgtgtactt catcaaattt
      601 gatgatgatt tccatatcta tgtctacgat ttggtaaaaa catctgcaga gaacctctac
      661 ttccaatcgc accatcatca ccaccatgat tacaaggatg acgacgataa gtgaggatcc
//
