LOCUS       sequence-140918-         451 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 451)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD16A
FEATURES             Location/Qualifiers
     source          1..451
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ggtccgcagt tcccaactcc ttccctgagg tggtagagct gaatgtcggg
      121 ggtcaagttt attttactcg ccattccaca ttgataagca tccctcattc cctcctgtgg
      181 aaaatgtttt ccccaaagag agacacggct aatgatctag ccaaggactc caagggaagg
      241 tttttcattg acagagatgg attcttgttc cgttatattc tggactatct cagggacagg
      301 caggtggtcc tgcctgatca ctttccagaa aaaggaagac tgaaaaggga agctgaatac
      361 ttccagctcc cagacttggt caaactcctg acccccgatg aaatcaagca aagcccagat
      421 gaatgacagt aaaggtggat acggatccga a
//
