LOCUS       sequence-14092-e        4338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pCSHSP:FLP
FEATURES             Location/Qualifiers
     source          1..4338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..31
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     13..30
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             72..1340
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     polyA_signal    1369..1503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1393..1412)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1447..1466
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1616..1636)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1616..1634)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1655..1671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1655..1671)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1668..1690)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1679..1695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1703..1709,1710..1727,1728..1733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1748..1769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1886..1903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2057..2645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2137..2156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2816..3607,3608..3676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3439..3458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3677..3781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3807..4262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3944..3965)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4156..4175
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 accggacgga tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat
       61 ccacagccac catgccacaa tttgatatat tatgtaaaac accacctaag gtcctggttc
      121 gtcagtttgt ggaaaggttt gaaagacctt caggggaaaa aatagcatca tgtgctgctg
      181 aactaaccta tttatgttgg atgattactc ataacggaac agcaatcaag agagccacat
      241 tcatgagcta taatactatc ataagcaatt cgctgagttt cgatattgtc aacaaatcac
      301 tccagtttaa atacaagacg caaaaagcaa caattctgga agcctcatta aagaaattaa
      361 ttcctgcttg ggaatttaca attattcctt acaatggaca aaaacatcaa tctgatatca
      421 ctgatattgt aagtagtttg caattacagt tcgaatcatc ggaagaagca gataagggaa
      481 atagccacag taaaaaaatg cttaaagcac ttctaagtga gggtgaaagc atctgggaga
      541 tcactgagaa aatactaaat tcgtttgagt atacctcgag atttacaaaa acaaaaactt
      601 tataccaatt cctcttccta gctactttca tcaattgtgg aagattcagc gatattaaga
      661 acgttgatcc gaaatcattt aaattagtcc aaaataagta tctgggagta ataatccagt
      721 gtttagtgac agagacaaag acaagcgtta gtaggcacat atacttcttt agcgcaaggg
      781 gtaggatcga tccacttgta tatttggatg aatttttgag gaattctgaa ccagtcctaa
      841 aacgagtaaa taggaccggc aattcttcaa gcaacaaaca ggaataccaa ttattaaaag
      901 ataacttagt cagatcgtac aacaaggctt tgaagaaaaa tgcgccttat ccaatctttg
      961 ctataaagaa tggcccaaaa tctcacattg gaagacattt gatgacctca tttctgtcaa
     1021 tgaagggcct aacggagttg actaatgttg tgggaaattg gagcgataag cgtgcttctg
     1081 ccgtggccag gacaacgtat actcatcaga taacagcaat acctgatcac tacttcgcac
     1141 tagtttctcg gtactatgca tatgatccaa tatcaaagga aatgatagca ttgaaggatg
     1201 agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa
     1261 gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat
     1321 cctacataaa tagacgcatt ctagaactat agtgagtcgt attacgtaga tccagacatg
     1381 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1441 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1501 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1561 ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt
     1621 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1681 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1741 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1801 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1861 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1921 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1981 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2041 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2101 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2161 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2221 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2281 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2341 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2401 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2461 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2521 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2581 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2641 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2701 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2761 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2821 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2881 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2941 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3001 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3061 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3781 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     3841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4261 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4321 gctattacgc cagtcgac
//
