LOCUS       sequence-140920-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STK10
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc gcgaatatga gcacgtccgc cgcgacctgg accccaacga ggtgtgggag
      121 atcgtgggcg agctgggcga cggcgccttc ggcaaggttt acaaggccaa gaataaggag
      181 acgggtgctt tggctgcggc caaagtcatt gaaaccaaga gtgaggagga gctggaggac
      241 tacatcgtgg agattgagat cctggccacc tgcgaccacc cctacattgt gaagctcctg
      301 ggagcctact atcacgacgg gaagctgtgg atcatgattg agttctgtcc agggggagcc
      361 gtggacgcca tcatgctgga gctggacaga ggcctcacgg agccccagat acaggtggtt
      421 tgccgccaga tgctagaagc cctcaacttc ctgcacagca agaggatcat ccaccgagat
      481 ctgaaagctg gcaacgtgct gatgaccctc gagggagaca tcaggctggc tgactttggt
      541 gtgtctgcca agaatctgaa gactctacag aaacgagatt ccttcatcgg cacgccttac
      601 tggatggccc ccgaggtggt catgtgtgag accatgaaag acacgcccta cgactacaaa
      661 gccgacatct ggtccctggg catcacgctg attgagatgg cccagatcga gccgccacac
      721 cacgagctca accccatgcg ggtcctgcta aagatcgcca agtcagaccc tcccacgctg
      781 ctcacgccct ccaagtggtc tgtagagttc cgtgacttcc tgaagatagc cctggataag
      841 aacccagaaa cccgacccag tgccgcgcag ctgctggagc atcccttcgt cagcagcatc
      901 accagtaaca aggctctgcg ggagctggtg gctgaggcca aggccgagtg acagtaaagg
      961 tggatacgga tccgaa
//
