LOCUS       sequence-140922-         433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 433)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD17
FEATURES             Location/Qualifiers
     source          1..433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gggcgggcgg ccgcgccgca ggcggctggg gcaagtgggt gcggctcaac
      121 gtggggggca cggtgttcct gaccacccgg cagacgctgt gccgcgagca gaagtccttc
      181 ctcagccgcc tgtgccaggg ggaagagctg cagtcggacc gggatgagac cggggcctac
      241 ctcattgacc gtgaccccac ctacttcggg cccatcctga acttcctccg gcatggcaag
      301 ctggtgctgg acaaggacat ggctgaggag ggggtcctgg aggaagccga gttctacaac
      361 atcggcccgc tgatccgcat catcaaagac cggatggaag agaagtgaca gtaaaggtgg
      421 atacggatcc gaa
//
