LOCUS       sequence-140926-         475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 475)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHIPA
FEATURES             Location/Qualifiers
     source          1..475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gctatgatat ccaggcgtgg aaaaaacagt gcgaagaact gctgaatctg
      121 atttttcagt gtgaagatag cgaaccgttt cgtcagccgg ttgatctgct ggaatatccg
      181 gattatcgcg atatcattga taccccgatg gattttgcga ccgtgcgcga aaccctggaa
      241 gcgggtaact atgaaagccc gatggaactg tgcaaagatg tgcgcctgat ttttagcaac
      301 agcaaagcct ataccccgag caaacgtagc cgtatctata gcatgagcct gcgtctgagc
      361 gcgttttttg aagaacacat tagcagcgtg ctgagcgatt ataaaagcgc gctgcgcttt
      421 cataaacgta ataccattac caaacgctga cagtaaaggt ggatacggat ccgaa
//
