LOCUS       sequence-140928-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pRK5-HA-Sestrin2-L261A
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..117
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcccaggtcc aactgcacct cggttctatc gattgaattc ccgaaccgac agtcggtctc
       61 ttcaccaagg ccattcgcgc caccatgggc tacccctatg atgtgcctga ctacgcagat
      121 ctcaatggtg gtggtggtgg gtcgacgatg atcgtggcgg actccgagtg ccgcgcagag
      181 ctcaaggact acctgcggtt cgccccgggc ggcgtcggcg actcgggccc cggagaggag
      241 cagagggaga gccgggctcg gcgaggccct cgagggccca gcgccttcat ccccgtggag
      301 gaggtccttc gggagggggc tgagagcctc gagcagcacc tggggctgga ggcactgatg
      361 tcctctgggc gagtagacaa cctggcagtg gtgatgggcc tgcaccctga ctactttacc
      421 agcttctggc gcctgcacta cctgctgctg cacacggatg gtcccttggc cagctcctgg
      481 cgccactaca ttgccatcat ggctgccgcc cgccatcagt gttcttacct ggtaggctcc
      541 cacatggccg agtttctgca gactggtggt gaccctgagt ggctgctggg cctccaccgg
      601 gcccccgaga agctgcgcaa actcagcgag atcaacaagt tgctggcgca tcggccatgg
      661 ctcatcacca aggaacacat ccaggccttg ctgaagaccg gcgagcacac ttggtccctg
      721 gccgagctca ttcaggctct ggtcctgctc acccactgcc actcgctctc ctccttcgtg
      781 tttggctgtg gcatcctccc tgagggggat gcagatggca gccctgcccc ccaggcacct
      841 acacccccta gtgaacagag cagcccccca agcagggacc cgttgaacaa ctctgggggc
      901 tttga
//
