LOCUS       sequence-140933-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agggtccaac tgcacctcgg ttctatcgat tgaattcacc atggaaatgg actacaagga
       61 tgacgatgac aagggtggtg gtggtggtgc gtcgacgatg atcgtggcgg actccgagtg
      121 ccgcgcagag ctcaaggact acctgcggtt cgccccgggc ggcgtcggcg actcgggccc
      181 cggagaggag cagagggaga gccgggctcg gcgaggccct cgagggccca gcgccttcat
      241 ccccgtggag gaggtccttc gggagggggc tgagagcctc gagcagcacc tggggctgga
      301 ggcactgatg tcctctgggc gagtagacaa cctggcagtg gtgatgggcc tgcaccctga
      361 ctactttacc agcttctggc gcctgcacta cctgctgctg cacacggatg gtcccttggc
      421 cagctcctgg cgccactaca ttgccatcat ggctgccgcc cgccatcagt gttcttacct
      481 ggtaggctcc cacatggccg agtttctgca gactggtggt gaccctgagt ggctgctggg
      541 cctccaccgg gcccccgaga agctgcgcaa actcagcgag atcaacaagt tgctggcgca
      601 tcggccatgg ctcatcacca aggaacacat ccaggccttg ctgaagaccg gcgagcacac
      661 ttggtccctg gccgagctca ttcaggctct ggtcctgctc acccactgcc actcgctctc
      721 ctccttcgtg tttggctgtg gcatcctccc tgagggggat gcagatggca gccctgcccc
      781 ccaggcacct acacccccta gtgaacagag cagcccccca agcagggacc cgttgaacaa
      841 ctctgggggc tttgagtctg cccgcgacgt gga
//
