LOCUS       sequence-140935-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pRK5-HA-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cccaggtcca actgcacctc ggttctatcg attgaattcc cgaaccgaca gtcggtctct
       61 tcaccaaggc cattcgcgcc accatgggct acccctatga tgtgcctgac tacgcagatc
      121 tcaatggtgg tggtggtggg tcgacgatga tcgtggcgga ctccgagtgc cgcgcagagc
      181 tcaaggacta cctgcggttc gccccgggcg gcgtcggcga ctcgggcccc ggagaggagc
      241 agagggagag ccgggctcgg cgaggccctc gagggcccag cgccttcatc cccgtggagg
      301 aggtccttcg ggagggggct gagagcctcg agcagcacct ggggctggag gcactgatgt
      361 cctctgggcg agtagacaac ctggcagtgg tgatgggcct gcaccctgac tactttacca
      421 gcttctggcg cctgcactac ctgctgctgc acacggatgg tcccttggcc agctcctggc
      481 gccactacat tgccatcatg gctgccgccc gccatcagtg ttcttacctg gtaggctccc
      541 acatggccga gtttctgcag actggtggtg accctgagtg gctgctgggc ctccaccggg
      601 cccccgagaa gctgcgcaaa ctcagcgaga tcaacaagtt gctggcgcat cggccatggc
      661 tcatcaccaa ggaacacatc caggccttgc tgaagaccgg cgagcacact tggtccctgg
      721 ccgagctcat tcaggctctg gtcctgctca cccactgcca ctcgctctcc tccttcgtgt
      781 ttggctgtgg catcctc
//
