LOCUS       sequence-140937-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pRK5-FLAG-dSestrin (CG11299-PD)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccaactgca cctcggttct atcgattgaa ttcaccatgg aaatggacta caaggatgac
       61 gatgacaagg gtggtggtgg tggtgcgtcg acgatggacg agctcgatga cctggaccag
      121 gtgacccaag tgattggata tcatccccag ttccacgatc acttcctggc cacccagaac
      181 ttcattatga agggcgatgg tccgctgcct aacgattaca gatactactt ggccataatt
      241 gctgctgccc gccatcagtg tccgtatctg gtgaagcggt acgagaagga gttcatcaac
      301 cagggcggcg atagtgcctg gctgggcggc ctggacttta tacccgccaa actacgtgcc
      361 atatacgata tcaataaaat attagcccat cggccctggc tgctgcgcaa ggagcatatc
      421 gagcggctca ccaaggggaa gaacagctgg tcgctgtcgg aggtggtgca cgccatggtc
      481 ctgctctcgc acttccactc gctgtcctcc tttgtgttct cctgcggcct cacacaaaag
      541 ctggacgggc tgtccagtcc caagctgaag agtccgcccg cagcagtggc ggccctcgca
      601 cccaccattc tcatcactcc cacctcgccg acggagccgc agaaggggaa gccagtgctg
      661 gcggagatct cgctcaacaa tgcaaatccg gattacgaca gccagaccgc agcgagcagt
      721 aatggaggag ctccaccgga ctcggcaaat gcggttgcgg atggacccga tgcaacgacc
      781 cttaacggat atttggccac ggcgcagcag ctgccgcagc agcacggtat cagtgtggag
      841 acgctgatgg agcgcatgaa ggtcctgtc
//
