LOCUS       sequence-14094-e        7338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pBSHSP:FLP;CMV:tdTomato-SceI
FEATURES             Location/Qualifiers
     source          1..7338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    776..910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(939..2207)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     promoter        complement(2248..2266)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        2285..3269
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3304..3322
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             3354..4784
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    4825..4959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5074..5090)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5074..5090)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5127..5147)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5127..5145)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5189..5205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5189..5205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5202..5224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5213..5229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5237..5243,5244..5261,5262..5267))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5282..5303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5420..5437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5591..6179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5671..5690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6350..7141,7142..7210))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6973..6992
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7211..7315)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattgggtac cgggcccaat gcattggcgc cgcggccgcg aattaaaaaa
      721 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      901 tgtctggatc tacgtaatac gactcactat agttctagaa tgcgtctatt tatgtaggat
      961 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     1021 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     1081 tccttcaatg ctatcatttc ctttgatatt ggatcatatg catagtaccg agaaactagt
     1141 gcgaagtagt gatcaggtat tgctgttatc tgatgagtat acgttgtcct ggccacggca
     1201 gaagcacgct tatcgctcca atttcccaca acattagtca actccgttag gcccttcatt
     1261 gacagaaatg aggtcatcaa atgtcttcca atgtgagatt ttgggccatt ctttatagca
     1321 aagattggat aaggcgcatt tttcttcaaa gccttgttgt acgatctgac taagttatct
     1381 tttaataatt ggtattcctg tttgttgctt gaagaattgc cggtcctatt tactcgtttt
     1441 aggactggtt cagaattcct caaaaattca tccaaatata caagtggatc gatcctaccc
     1501 cttgcgctaa agaagtatat gtgcctacta acgcttgtct ttgtctctgt cactaaacac
     1561 tggattatta ctcccagata cttattttgg actaatttaa atgatttcgg atcaacgttc
     1621 ttaatatcgc tgaatcttcc acaattgatg aaagtagcta ggaagaggaa ttggtataaa
     1681 gtttttgttt ttgtaaatct cgaggtatac tcaaacgaat ttagtatttt ctcagtgatc
     1741 tcccagatgc tttcaccctc acttagaagt gctttaagca tttttttact gtggctattt
     1801 cccttatctg cttcttccga tgattcgaac tgtaattgca aactacttac aatatcagtg
     1861 atatcagatt gatgtttttg tccattgtaa ggaataattg taaattccca agcaggaatt
     1921 aatttcttta atgaggcttc cagaattgtt gctttttgcg tcttgtattt aaactggagt
     1981 gatttgttga caatatcgaa actcagcgaa ttgcttatga tagtattata gctcatgaat
     2041 gtggctctct tgattgctgt tccgttatga gtaatcatcc aacataaata ggttagttca
     2101 gcagcacatg atgctatttt ttcccctgaa ggtctttcaa acctttccac aaactgacga
     2161 accaggacct taggtggtgt tttacataat atatcaaatt gtggcatggt ggctgtggat
     2221 cctgcaaaaa gaacaagtag cttgtattct atagtgtcac ctaaatcatc cgtccggtnn
     2281 ngtcgaccat agccaattca atatggcgta tatggactca tgccaattca atatggtgga
     2341 tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga
     2401 tctggacccc agccaattca atatggcgga cttggcacca tgccaattca atatggcgga
     2461 cttggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg
     2521 tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat
     2581 attggctata tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg
     2641 gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg
     2701 gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg
     2761 cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct
     2821 ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt
     2881 caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc
     2941 attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac
     3001 gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc
     3061 aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat tgacgtcaat
     3121 ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg
     3181 caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg
     3241 tctatataag caatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc
     3301 ttgatttagg tgacactata gaatacaagc tacttgttct ttttgcagga tccatggtga
     3361 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     3421 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3481 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     3541 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     3601 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3661 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     3721 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     3781 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     3841 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3901 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     3961 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     4021 acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct
     4081 ccgaggacaa caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
     4141 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4201 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     4261 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc
     4321 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4381 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
     4441 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
     4501 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     4561 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca
     4621 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg
     4681 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
     4741 gccaccacct gttcctgtac ggcatggacg agctgtacaa gtaagagctc gagcctctag
     4801 aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga
     4861 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4921 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4981 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgcggtatcg
     5041 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca
     5101 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cattaccctg
     5161 ttatccctac gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5221 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5281 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5341 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5401 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5461 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5521 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5581 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5641 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5701 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5761 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5821 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5881 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5941 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6001 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6061 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6121 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6181 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6241 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6301 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6361 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6421 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6481 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6541 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6601 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6661 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6721 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6781 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6841 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6901 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6961 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7021 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7081 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7141 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7201 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7261 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7321 ttccccgaaa agtgccac
//
