LOCUS       sequence-140940-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, 
            nourseothricin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS288 C terminal mKO2 natR
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..732
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
ORIGIN
        1 atacactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaggtgct
       61 ggatctggtt cattaattaa catggtttct gttattaagc ctgaaatgaa gatgcgttac
      121 tatatggatg gttctgttaa tggtcatgaa tttactattg aaggtgaagg tactggtcgt
      181 ccttatgaag gtcatcaaga aatgactctt cgtgttacta tggctgaagg tggtcctatg
      241 ccttttgctt ttgatcttgt ttctcatgtt ttttgctatg gtcatcgtgt ttttactaag
      301 tatcctgaag aaattcctga ttactttaag caagcttttc ctgaaggtct ttcttgggaa
      361 cgttctcttg aatttgaaga tggtggttct gcttctgttt ctgctcatat ttctcttcgt
      421 ggtaatactt tttaccataa gtctaagttt actggtgtta attttcctgc tgatggtcct
      481 attatgcaaa atcaatctgt tgattgggaa ccttctactg aaaagattac tgcttctgat
      541 ggtgttctta agggtgatgt tacaatgtac cttaagttag aaggtggtgg taaccataag
      601 tgccaaatga agactactta caaggctgct aaagaaattc ttgaaatgcc tggtgatcat
      661 tacattggac atcgtcttgt tcgtaagact gaaggtaaca ttactgaaca agttgaagat
      721 gctgttgctc attattctta aaaaaagact aatgtaaaat ttttttggtt ggttattgaa
      781 aaagtcgatg ccttgtttgc gtttgttttc ctaggcgttt tatgtcagaa ggcatttaga
      841 attagtatac aagtactctt tggtaaaatt ttatgtagcg actaaaatat taactattat
      901 agataaacac cttggg
//
