LOCUS       sequence-140947-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pLJM1-FLAG-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttagtgaac cgtcagatcc gcctagcgct accggtgcca ccatggacta caaggatgac
       61 gatgacaagg gtggtggtgg tggtgcgtcg acgatgatcg tggcggactc cgagtgccgc
      121 gcagagctca aggactacct gcggttcgcc ccgggcggcg tcggcgactc gggccccgga
      181 gaggagcaga gggagagccg ggctcggcga ggccctcgag ggcccagcgc cttcatcccc
      241 gtggaggagg tccttcggga gggggctgag agcctcgagc agcacctggg gctggaggca
      301 ctgatgtcct ctgggcgagt agacaacctg gcagtggtga tgggcctgca ccctgactac
      361 tttaccagct tctggcgcct gcactacctg ctgctgcaca cggatggtcc cttggccagc
      421 tcctggcgcc actacattgc catcatggct gccgcccgcc atcagtgttc ttacctggta
      481 ggctcccaca tggccgagtt tctgcagact ggtggtgacc ctgagtggct gctgggcctc
      541 caccgggccc ccgagaagct gcgcaaactc agcgagatca acaagttgct ggcgcatcgg
      601 ccatggctca tcaccaagga acacatccag gccttgctga agaccggcga gcacacttgg
      661 tccctggccg agctcattca ggctctggtc ctgctcaccc actgccactc gctctcctcc
      721 ttcgtgtttg gctgtggcat cctccctgag ggggatgcag atggcagccc tgccccccag
      781 gcacctacac cccctagtga acagagcagc cccccaagca gggacccgtt gaacaactct
      841 gggggctttg agtctgcccg cgacgtggag gcgctgatgg a
//
