LOCUS       sequence-140948-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, 
            nourseothricin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS288 C terminal mKO2 natR
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     65..81
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     66..82
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(104..301)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(307..879)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg taccgggccc
       61 cccctcgagg tcgacggtat cgataagctt gatatgttta aaccagtata gcgaccagca
      121 ttcacatacg attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga
      181 tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta
      241 caatataaaa aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag
      301 tactgattag gggcagggca tgctcatgta gagcgcctgc tcgccgtccg aggcggtgcc
      361 gtcgtacagg gcggtgtcca ggccgcagag ggtgaacccc atccgccggt acgcgtggat
      421 cgccggtgcg ttgacgttgg tgacctccag ccagaggtgc ccggcacccc gctcgcgggc
      481 gaactccgtc gcgagcccca tcaacgcgcg cccgaccccg tgcccccggt gctccggggc
      541 gacctcgatg tcctcgacgg tcagccggcg gttccagccg gagtacgaga cgaccacgaa
      601 gcccgccagg tcgccgtcgt ccccgtacgc gacgaacgtc cgagagtccg ggtcgccgtc
      661 ctccccgtcg tccgattcgt cgtccgattc gtcgtcgggg aacaccttgg tcaggggcgg
      721 gtccaccggc acctcccgca gggtgaagcc gtccccggtg gcggtgacgc ggaagacggt
      781 gtcggtggtg aaggacccat ccagtgcctc gatggcctcg gcgtcccccg ggacactggt
      841 gcggtaccgg taagccgtgt cgtcaagagt ggtacccatg gttgtttatg ttcggatgtg
      901 atgtgagaac tgtatcctag caagatttaa aaaggaagta tatgaaagaa gaacctcagt
      961 ggcaaatcct aaccttttat atttctctac aggg
//
