LOCUS       sequence-14095-e        4100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pCSHSP:Cre
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..31
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     13..30
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             68..1096
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    1131..1265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1155..1174)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1209..1228
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1378..1398)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1378..1396)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1417..1433)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1417..1433)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1430..1452)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1441..1457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1465..1471,1472..1489,1490..1495))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1510..1531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1648..1665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1819..2407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1899..1918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2578..3369,3370..3438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3201..3220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3439..3543)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3569..4024)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3706..3727)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3918..3937
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 accggacgga tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat
       61 ctccgatatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
      121 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
      181 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
      241 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
      301 tcaggcgcgc ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct
      361 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
      421 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
      481 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
      541 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
      601 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
      661 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
      721 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
      781 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
      841 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
      901 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
      961 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
     1021 aaatattgtc atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
     1081 gctggaagat ggcgatggtt ctctagaact atagtgagtc gtattacgta gatccagaca
     1141 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1201 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1261 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1321 ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct
     1381 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1441 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1501 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1561 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1621 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1681 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1741 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1801 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1861 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1921 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1981 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2041 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2101 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2161 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2221 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2281 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2341 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2401 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2461 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2521 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2641 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2701 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2881 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3421 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3481 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3541 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     3601 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3661 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3721 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3781 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3841 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3901 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3961 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4021 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4081 tcgctattac gccagtcgac
//
