LOCUS       sequence-140955-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pS2-FLAG-Sestrin2-L261A
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             184..417
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    489..610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(526..545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     580..599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      650..785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(805..824)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(806..824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(844..866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
       61 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcggtacctt
      121 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
      181 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
      241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
      421 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
      481 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      601 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
      661 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      721 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      781 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactgggc
      841 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta at
//
