LOCUS       sequence-140958-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pS2-FLAG-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        82..592
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     511..529
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(614..633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 attacaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
       61 ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca gccctgggtt
      121 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
      181 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
      241 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
      301 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
      361 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
      421 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
      481 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
      541 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc
      601 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      661 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      721 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      781 cgtcgggctc gacatcggca a
//
