LOCUS       sequence-140959-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS289 C terminal E2C G418R
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     64..80
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(102..299)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc
       61 cctcgaggtc gacggtatcg ataagcttga tatgtttaaa ccagtatagc gaccagcatt
      121 cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg
      181 atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca
      241 atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta
      301 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
      361 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
      421 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
      481 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
      541 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
      601 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
      661 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
      721 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
      781 gatattcttc taatacctgg aatgctgttt tgccggggat cgcagtggtg agtaaccatg
      841 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
      901 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
      961 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     1021 cgacattatc gcgagcccat tt
//
