LOCUS       sequence-14096-e        7100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pBSHSP:Cre;CMV:tdTomato-SceI
FEATURES             Location/Qualifiers
     source          1..7100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    776..910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(945..1973)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     promoter        complement(2010..2028)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        2047..3031
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3066..3084
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             3116..4546
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    4587..4721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4836..4852)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4836..4852)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4889..4909)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4889..4907)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4951..4967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4951..4967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4964..4986)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4975..4991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4999..5005,5006..5023,5024..5029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5044..5065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5182..5199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5353..5941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5433..5452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6112..6903,6904..6972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6735..6754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6973..7077)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattgggtac cgggcccaat gcattggcgc cgcggccgcg aattaaaaaa
      721 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      781 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      841 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
      901 tgtctggatc tacgtaatac gactcactat agttctagag aaccatcgcc atcttccagc
      961 aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt
     1021 acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata
     1081 tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag
     1141 tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga
     1201 gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac
     1261 aggtagttat tcggatcatc agctacacca gagacggaaa tccatcgctc gaccagttta
     1321 gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg
     1381 ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac cctgatcctg
     1441 gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt
     1501 atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt
     1561 tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc
     1621 cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga
     1681 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga
     1741 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc
     1801 aacttgcacc atgccgccca cgaccggcaa acggacagaa gcattttcca ggtatgctca
     1861 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt
     1921 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga catatcggag
     1981 atcctgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatca tccgtccggt
     2041 nnngtcgacc atagccaatt caatatggcg tatatggact catgccaatt caatatggtg
     2101 gatctggacc tgtgccaatt caatatggcg tatatggact cgtgccaatt caatatggtg
     2161 gatctggacc ccagccaatt caatatggcg gacttggcac catgccaatt caatatggcg
     2221 gacttggcac tgtgccaact ggggaggggt ctacttggca cggtgccaag tttgaggagg
     2281 ggtcttggcc ctgtgccaag tccgccatat tgaattggca tggtgccaat aatggcggcc
     2341 atattggcta tatgccagga tcaatatata ggcaatatcc aatatggccc tatgccaata
     2401 tggctattgg ccaggttcaa tactatgtat tggccctatg ccatatagta ttccatatat
     2461 gggttttcct attgacgtag atagcccctc ccaatgggcg gtcccatata ccatatatgg
     2521 ggcttcctaa taccgcccat agccactccc ccattgacgt caatggtctc tatatatggt
     2581 ctttcctatt gacgtcatat gggcggtcct attgacgtat atggcgcctc ccccattgac
     2641 gtcaattacg gtaaatggcc cgcctggctc aatgcccatt gacgtcaata ggaccaccca
     2701 ccattgacgt caatgggatg gctcattgcc cattcatatc cgttctcacg ccccctattg
     2761 acgtcaatga cggtaaatgg cccacttggc agtacatcaa tatctattaa tagtaacttg
     2821 gcaagtacat tactattgga agtacgccag ggtacattgg cagtactccc attgacgtca
     2881 atggcggtaa atggcccgcg atggctgcca agtacatccc cattgacgtc aatggggagg
     2941 ggcaatgacg caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg cctaatggga
     3001 ggtctatata agcaatgctc gtttagggaa ccgccattct gcctggggac gtcggagcaa
     3061 gcttgattta ggtgacacta tagaatacaa gctacttgtt ctttttgcag gatccatggt
     3121 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     3181 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3241 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3301 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     3361 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3421 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     3481 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     3541 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     3601 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     3661 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     3721 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     3781 ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc
     3841 ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     3901 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3961 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     4021 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     4081 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4141 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     4201 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     4261 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     4321 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     4381 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     4441 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     4501 ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaagagc tcgagcctct
     4561 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     4621 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4681 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4741 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggtat
     4801 cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc
     4861 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcattaccc
     4921 tgttatccct acgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4981 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5041 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5101 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5161 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5221 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5281 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5341 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5401 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5461 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5521 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5581 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5641 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5701 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5761 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     5821 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5881 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5941 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6001 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6061 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6121 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6181 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6241 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6301 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6361 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6421 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6481 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6541 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6601 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6661 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6721 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6781 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6841 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6901 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6961 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7021 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7081 atttccccga aaagtgccac
//
