LOCUS       sequence-140960-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Wolfson RL, Chantranupong L, Saxton RA, Shen K, Scaria SM, Cantor 
            JR, Sabatini DM
  TITLE     Sestrin2 is a leucine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2016 Jan 1;351(6268):43-8. doi: 10.1126/science.aab2674. 
            Epub 2015 Oct 8.
  PUBMED    26449471
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab2674"; journalName: "Science"; date: "2016-01-1-
            1"; volume: "351"; issue: "6268"; pages: "43-8"
COMMENT     Sequence Label: pS2-FLAG-Sestrin2-E451A
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             181..414
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    486..607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(523..542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     577..596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
       61 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa
      121 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
      181 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
      421 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
      481 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      541 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      601 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
      661 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
      721 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct ttt
//
