LOCUS       sequence-140961-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS291 C terminal mKO2 kanR
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..728
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
ORIGIN
        1 actcactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga ggtgctggat
       61 ctggttcatt aattaacatg gtttctgtta ttaagcctga aatgaagatg cgttactata
      121 tggatggttc tgttaatggt catgaattta ctattgaagg tgaaggtact ggtcgtcctt
      181 atgaaggtca tcaagaaatg actcttcgtg ttactatggc tgaaggtggt cctatgcctt
      241 ttgcttttga tcttgtttct catgtttttt gctatggtca tcgtgttttt actaagtatc
      301 ctgaagaaat tcctgattac tttaagcaag cttttcctga aggtctttct tgggaacgtt
      361 ctcttgaatt tgaagatggt ggttctgctt ctgtttctgc tcatatttct cttcgtggta
      421 atacttttta ccataagtct aagtttactg gtgttaattt tcctgctgat ggtcctatta
      481 tgcaaaatca atctgttgat tgggaacctt ctactgaaaa gattactgct tctgatggtg
      541 ttcttaaggg tgatgttaca atgtacctta agttagaagg tggtggtaac cataagtgcc
      601 aaatgaagac tacttacaag gctgctaaag aaattcttga aatgcctggt gatcattaca
      661 ttggacatcg tcttgttcgt aagactgaag gtaacattac tgaacaagtt gaagatgctg
      721 ttgctcatta ttcttaaaaa aagactaatg taaaa
//
