LOCUS       sequence-140962-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS291 C terminal mKO2 kanR
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..78
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     63..79
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(101..298)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 attacgccaa gctcgaaata accctcacta aagggaacaa aagctggtac cgggcccccc
       61 ctcgaggtcg acggtatcga taagcttgat atgtttaaac cagtatagcg accagcattc
      121 acatacgatt gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga
      181 tgtcgaggcg aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa
      241 tataaaaaaa ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac
      301 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
      361 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
      421 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
      481 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
      541 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
      601 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
      661 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
      721 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
      781 atattcttct aatacctgga atgctgtttt gccggggatc gcagtggtga gtaaccatgc
      841 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
      901 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
      961 aa
//
