LOCUS       sequence-140963-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            hygB resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS287 C terminal E2C hygBR
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     64..80
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(102..299)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc
       61 cctcgaggtc gacggtatcg ataagcttga tatgtttaaa ccagtatagc gaccagcatt
      121 cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg
      181 atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca
      241 atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta
      301 ctgattattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
      361 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
      421 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
      481 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
      541 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
      601 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
      661 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
      721 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
      781 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
      841 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
      901 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
      961 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     1021 gcaggtcttg caacgtgaca ccctgtgcac ggcggg
//
