LOCUS       sequence-140965-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV 
            promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV Puro DEST (w118-1)
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..265)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(349..373)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(370..573)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(399..419)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        574..877
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcagcttt tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagag
      301 tcggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt
      361 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
      421 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
      481 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
      541 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
      601 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
      661 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
      721 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
      781 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
      841 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactcccaag cttatcgata
      901 aaa
//
