LOCUS       sequence-140986-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 1 Destination vector - Position C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU1-C-lacZ
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             381..392
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(401..417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(401..417)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(414..436)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    425..441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    478..497
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      500..543
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(547..565)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        633..737
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcagtgagcg aggaagcctg cagcgtctct cttcaacatg agaccgcatg cggatccact
       61 agtgcgaaaa aaccccgccc tgtcaggggc ggggtttttt gcgttagttg ttagacagcg
      121 gcggcagcag ccaagacagt ttacgttccg gtttggtcag cagacggatc agagaacgca
      181 gctgctggga cggacggtcg gtacgagctt cttcagagtt acgccaagaa gcgaacggcg
      241 ggtgagcagc cagacggttc agctgggtaa cacccgggtt ttcccagtca cgacgttgca
      301 gaacaacagc cagagagtta cccggagaag aagagatagc gtgcggaact tccagcagca
      361 gtttcgggtc acccatgtcc tttctcctct ttgtaccatg gtcatagctg tttcctgtgt
      421 gaaattgtta tccgctcaca acatatgtct agaggtctct atagccggtg agacgttcta
      481 gaagcggccg cgaattccag aaatcatcct tagcgaaagc taaggatttt ttttatctga
      541 aattctgcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      601 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      841 aagatcagtt gg
//
