LOCUS       sequence-140993-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Secondary module (BpiI) site in pTU2-a backbone with colE1 
            (medium-copy) origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2S-a (colE1 origin)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    197..218
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      271..328
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(352..369)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      121 cttacgtgcc cgatcaactc gagtgccacc tgacgtccta taagtcttcc atatgcacgc
      181 atgcgaagac aagtacgaat tcgcggccgc ttctagaggt ctcactatat ctagagacgc
      241 accgtctcac cgggtacaga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc
      301 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
      361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      421 aatacggtta tccacagaat caggggataa cgcaggaaag ggatctggat ctaggtgaag
      481 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtctta
//
