LOCUS       sequence-140996-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            nourseothricin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS290 C terminal E2C natR
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     63..79
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     64..80
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(102..299)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(305..877)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc
       61 cctcgaggtc gacggtatcg ataagcttga tatgtttaaa ccagtatagc gaccagcatt
      121 cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg
      181 atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca
      241 atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta
      301 ctgattaggg gcagggcatg ctcatgtaga gcgcctgctc gccgtccgag gcggtgccgt
      361 cgtacagggc ggtgtccagg ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg
      421 ccggtgcgtt gacgttggtg acctccagcc agaggtgccc ggcaccccgc tcgcgggcga
      481 actccgtcgc gagccccatc aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga
      541 cctcgatgtc ctcgacggtc agccggcggt tccagccgga gtacgagacg accacgaagc
      601 ccgccaggtc gccgtcgtcc ccgtacgcga cgaacgtccg agagtccggg tcgccgtcct
      661 ccccgtcgtc cgattcgtcg tccgattcgt cgtcggggaa caccttggtc aggggcgggt
      721 ccaccggcac ctcccgcagg gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt
      781 cggtggtgaa ggacccatcc agtgcctcga tggcctcggc gtcccccggg acactggtgc
      841 ggtaccggta agccgtgtcg tcaagagtgg tacccatggt tgtttatgtt cggatgtgat
      901 gtgagaactg tatcctagca agatttaaaa aggaagtata tgaaagaaga acctcagtgg
      961 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
     1021 tgtgaggg
//
