LOCUS       sequence-140998-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTU2-A backbone, with RFP negative selection and colE1 (medium-copy)
            origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU2-A-RFP (colE1 origin)
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     199..217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(221..264)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    267..288
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    422..443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(458..463,464..481,482..488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    496..512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             530..541
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
      121 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
      181 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
      241 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagaggt ctcactatat
      301 ctagagacgt ctagacatat gcaatacgca aaccgcctct ccccgcgcgt tggccgattc
      361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      421 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      481 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa
      541 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
      601 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
      661 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
      721 ggacatcctg tccccgcagt tccagtacgg ttcca
//
