LOCUS       sequence-141001-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L-Lactate inducible promoter from E. coli MG1655.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-Bba_K822000
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      483..540
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(564..581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc atatgggtct cactatcaca
      121 ttcctatagg ccgagtaagg tgttcacgcc gcatccggca agataaggcg ctctggatca
      181 acaacctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc
      241 acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa tttcccaatt
      301 aaaactcaca tcaatgttgc caatacataa catttagtta accattcatt gtcattatcc
      361 ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca
      421 gactcattac acgatgtgcg tggactccgt acagagaccg catgccgtct cattagctgc
      481 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
      541 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      721 gcgttgctgg cgtttttcca caggctccgc ccc
//
