LOCUS       sequence-141044-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combined sgRNA/Cas9 vector with LEU2 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     sgRNA/Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMZ377
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     645..667
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     659..676
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     660..676
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(726..777)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 tttttatgat atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa
       61 ttacaaaatg gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt
      121 atcaagaagg agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg
      181 ctcaacgtga taaggaaaaa gaattgcact ttaacattaa tattgacaag gaggagggca
      241 ccacacaaaa agttaggtgt aacagaaaat catgaaacta tgattcctaa tttatatatt
      301 ggaggatttt ctctaaaaaa aaaaaaatac aacaaataaa aaacactcaa tgacctgacc
      361 atttgatgga gtttaagtca ataccttctt gaaccatttc ccataatggt gaaagttccc
      421 tcaagaattt tactctgtca gaaacggcct taacgacgta gtcgatcgac ggtgtgaaat
      481 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
      541 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      601 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      661 taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gactctagag gatccccacg
      721 gtacccctgt ttcgtcctca cggactcatc agaccggaaa gcacatccgg tgacagggca
      781 ccaccgactc ggtgccactc acttttttca agttgataac ggactagcct tattttaact
      841 tgctatttct agctctaaaa cagagagata acccacaaga attgagttaa gcccattctt
//
