LOCUS       sequence-141045-       11906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Combined sgRNA/Cas9 vector with LEU2 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11906)
  TITLE     sgRNA/Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMZ377
FEATURES             Location/Qualifiers
     source          1..11906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      539..1320
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     1429..1445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1696..2103
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             2104..3198
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     3870..3886
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(3936..3987)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(4106..4462)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(4499..4515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4499..4515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4512..4534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4523..4539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4547..4553,4554..4571,4572..4577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4592..4613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4730..4747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4901..5489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4981..5000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5660..6451,6452..6520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6283..6302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6521..6625)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6654..7395
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             7416..11519
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7416..11519
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7416..11519
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11532..11552
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11585..11832
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(11601..11619)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     11879..11897
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataatcg atggccaacc ttccaattca ttaaatcgag tcctccatca gatgatttac
      241 aaggttttgc atagaatccc taagtaagag aagttgttag tgatttcttc aagtttacta
      301 aaatttacat acaaatggat gatctcttct ccattgtttc ctttcttctt gcaaacgcgt
      361 tttacaaaga gatgacatgc tattttctga ttattttttt ctatagtttt ctattttgta
      421 cgtccttggg agtccgaaat gtaaaatcgg ggtatggtta gtagcggcag cgttcattat
      481 ggtatataaa agataaaaag taggggcaag cgagacagaa tgggatacaa gggcatcgtc
      541 tataattata gctaaaaatt gtattttaat ttgtattttt tgtaatttta tttctcattg
      601 ttttacttaa aatgaatgcg aattagagaa aacttcaacg aaatgtcaaa ataagctcag
      661 caaaatatac aattttaggg aaagcgatca gcaacttatt ctccgttgta gattcttttt
      721 cttcataaat tccaacaatg taactgccaa ttgatttttc tctagatgct aataaattga
      781 aactaatgaa tataataaca caggttttga taaattgtaa actcttactc aaaaaaagaa
      841 tgttaaaact aatttaacct ttttataaag gctcatggcg ttaaagagga tagtaaaaat
      901 tctacaattt tttaatttaa tgtcacaatt gtcttgcgaa tataattcta ttaattttcg
      961 ctaactgctg ttttagtact attactttca atatgcctta tatgcaactt taattcatga
     1021 agaatgcaat atttttgcaa tctttcttgt actgagcgct ttttatcatt tagtactcat
     1081 tatttaattt ttcagtaaag ggcaataaat aaatttgtag aagatgcaat gtaatctcct
     1141 ctatcctttt gctcatatgt ttatgagtat acctagtcta gaaaggcttg tatttaaaat
     1201 attattcaat aaaaattcac aatttttacg acatgtgcta ccattcactt aacttcctga
     1261 ttataaaatt ggttcgttta tactaattac ttaagtacct ttaactaaac aaaatgccta
     1321 tatatatatt aatttacaat gagtgtcaga taagtcacta tgtccgagtg gttaaggagt
     1381 tagactcgcc atcgtgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1441 ccagtgccaa gcttgtcgag gagaacttct agtatatcta catacctaat attattgcct
     1501 tattaaaaat ggaatcccaa caattacatc aaaatccaca ttctcttcaa aatcaattgt
     1561 cctgtacttc cttgttcatg tgtgttcaaa aacgttatat ttataggata attatactct
     1621 atttctcaac aagtaattgg ttgtttggcc gagcggtcta aggcgcctga ttcaagaaat
     1681 atcttgaccg cagttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc
     1741 tcttagcaac cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac
     1801 agaatcaaat tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata
     1861 cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga gagccgcgga accggctttt
     1921 catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca
     1981 atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta
     2041 acttttctta ccttttacat ttcagcaata tatatatata tatttcaagg atataccatt
     2101 ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc
     2161 acagccgaag ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc
     2221 gatttcgaaa atcatttaat tggtggtgct gctatcgatg ctacaggtgt tccacttcca
     2281 gatgaggcgc tggaagcctc caagaaggtt gatgccgttt tgttaggtgc tgtgggtggt
     2341 cctaaatggg gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa
     2401 cttcaattgt acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta
     2461 tctccaatca agccacaatt tgctaaaggt actgacttcg ttgttgtcag agaattagtg
     2521 ggaggtattt actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt
     2581 gaacaataca ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta
     2641 caacatgagc caccattgcc tatttggtcc ttggataaag ctaatgtttt ggcctcttca
     2701 agattatgga gaaaaactgt ggaggaaacc atcaagaacg aattccctac attgaaggtt
     2761 caacatcaat tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat
     2821 ggtattataa tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc
     2881 ccaggttcct tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc
     2941 gcatttggtt tgtacgaacc atgccacggt tctgctccag atttgccaaa gaataaggtc
     3001 aaccctatcg ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct
     3061 gaagaaggta aggccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact
     3121 ggtgatttag gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt
     3181 aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata
     3241 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga
     3301 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag
     3361 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact
     3421 ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt aacagaaaat
     3481 catgaaacta tgattcctaa tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac
     3541 aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca ataccttctt
     3601 gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca gaaacggcct
     3661 taacgacgta gtcgatcgac ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     3721 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     3781 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     3841 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
     3901 cctgcaggtc gactctagag gatccccacg gtacccctgt ttcgtcctca cggactcatc
     3961 agaccggaaa gcacatccgg tgacagggca ccaccgactc ggtgccactc acttttttca
     4021 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa cagagagata
     4081 acccacaaga attgagttaa gcccattctt cggtacaggt tatgtttttt ggcaacagca
     4141 actattcaaa tcaactgaaa gttttttaga attcaacaac ttccaatact caagtgtcca
     4201 agctagcaaa ctgtttaata atcgtgaccc aacattcctt tcatgcgcac cctcagcgtc
     4261 acataaatca acactttaca acacctattc aaagcttcta tatgccttcg tcatttaaat
     4321 gtctaacatt tcaaaaaagt gtttgctaat ggtaaattct tgagttttct tggaagcaca
     4381 tatataacga gtgtgactgt tctacgctcc ttgctgacta gtcatcagtc tacgcatgcc
     4441 aactaccacc aacataagca aaaggtaccc ggggtaccga gctcgaattc gtaatcatgt
     4501 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4561 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4621 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4681 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4741 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4801 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4861 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4921 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4981 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5041 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5101 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5161 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5221 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5281 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5341 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5401 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5461 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5521 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5581 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5641 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5701 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5761 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5821 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5881 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5941 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6001 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6061 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6121 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6181 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6241 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6301 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6361 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6421 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6481 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6541 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6601 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgccctac
     6661 aacaactaag aaaatggcta tcatgcggaa gctggtgaga agaacagcat cgggacaagg
     6721 gaaggaagaa caaagacaaa gaaaacaaaa gaaagcaatt gaaaacaaaa caaaacaatt
     6781 ttcattcctt ctcttatcat tccttttctt ttcttttctc tcattcaacg cactccatcg
     6841 tatccgtatt cctcttattt tttctctttc tctatatcca tttctttctc tctaggtgtg
     6901 tcctctctct ctcttcaatt tctctactcc gcattccaac gcatccttcc cccaacctcc
     6961 catttcctcc ttacggcccg atagcgatcg tctttccctc gctatcactc gctaccggcc
     7021 cctcctctgc accgtaacct cctacgtatt taccatatca taaagttttt tccgacgctt
     7081 atcgctgacc ccctgtcgcc ctcctattgg cttccggatt atcttcttgt ccataaggtg
     7141 atccatgctt cctgaagatt cccgaaatgt gtccactttg gcggggaatc attccatcca
     7201 cttctttctc tctcgctttc ctcattcggc gctccccttc cgcgtctcat tggtcttccg
     7261 ctccgttttt gctttgccga tgttacttgg ggagaggtgc gataatcctt tcgcaaaaac
     7321 tcggtttgac gcctcccatg gtataaatag tgggtggtgg acaggtgcct tcgcttttct
     7381 ttaagcaaga gaattgctgc aggtcgactc tagagatgga caagaagtac tccattgggc
     7441 tcgatatcgg cacaaacagc gtcggttggg ccgtcattac ggacgagtac aaggtgccga
     7501 gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg
     7561 gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac
     7621 ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg
     7681 agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg
     7741 aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc
     7801 atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg
     7861 ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc
     7921 tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg
     7981 ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca
     8041 aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc
     8101 tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca ctcgggctga
     8161 cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag
     8221 acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag tacgcagacc
     8281 tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt ctgcgagtga
     8341 acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat gatgagcacc
     8401 accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag aagtacaagg
     8461 aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc ggagcaagcc
     8521 aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc accgaggagc
     8581 tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa
     8641 gcatccccca ccagattcac ctgggcgaac tgcacgctat cctcaggcgg caagaggatt
     8701 tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca tttcggatac
     8761 cctactatgt aggccccctc gcccggggaa attccagatt cgcgtggatg actcgcaaat
     8821 cagaagagac catcactccc tggaacttcg aggaagtcgt ggataagggg gcctctgccc
     8881 agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa aaggtgcttc
     8941 ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc aaggtcaaat
     9001 acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag aaagctatcg
     9061 tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa gaagactatt
     9121 tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat cgcttcaacg
     9181 catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac ttcctggaca
     9241 atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg tttgaagata
     9301 gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac aaagtcatga
     9361 aacagctcaa gaggcgccga tatacaggat gggggcggct gtcaagaaaa ctgatcaatg
     9421 ggatccgaga taagcagtct ggaaagacaa tcctggattt tcttaagtcc gatggatttg
     9481 ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag gaggacatcc
     9541 agaaagcaca agtttctggc cagggggaca gtcttcacga gcacatcgct aatcttgcag
     9601 gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca
     9661 aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa
     9721 ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa
     9781 aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg
     9841 agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg
     9901 acatcaatcg gctctccgac tacgacgtgg atcatatcgt gccccagtct tttctcaaag
     9961 atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata
    10021 acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg
    10081 ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt
    10141 ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca
    10201 agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa aatgacaaac
    10261 tgattcgaga ggtgaaagtt attactctga agtctaagct ggtctcagat ttcagaaagg
    10321 actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat gatgcctacc
    10381 tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagcttgaa tctgaatttg
    10441 tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa
    10501 taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt ttcaagaccg
    10561 agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca aacggagaaa
    10621 caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccggaag gtcctgtcca
    10681 tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc tccaaggaaa
    10741 gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat tgggacccca
    10801 agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt gtggccaaag
    10861 tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc atcacaatca
    10921 tggagcgatc aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa ggatataaag
    10981 aggtcaaaaa agacctcatc attaagcttc ccaagtactc tctctttgag cttgaaaacg
    11041 gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag ctggcactgc
    11101 cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc aaagggtctc
    11161 ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac cttgatgaga
    11221 tcatcgagca aataagcgaa ttctccaaaa gagtgatcct cgccgacgct aacctcgata
    11281 aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag gcagaaaaca
    11341 ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag tacttcgaca
    11401 ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc
    11461 atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagaca
    11521 gcagggctga ccccaagaag aagaggaagg tgtgattttg gacctcgagt cattggacct
    11581 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
    11641 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
    11701 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
    11761 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
    11821 gctttaattt gcgatccccg tctaagaaac cattattatc atgacattaa cctataaaaa
    11881 taggcgtatc acgaggccct ttcgtc
//
