LOCUS       sequence-141048-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combined sgRNA/Cas9 vector with KanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     sgRNA/Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMZ376
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(29..80)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(199..555)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(592..608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(592..608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(605..627)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    616..632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(640..646,647..664,665..670))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    685..706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(823..840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcgactcta gaggatcccc acggtacccc tgtttcgtcc tcacggactc atcagaccgg
       61 aaagcacatc cggtgacagg gcaccaccga ctcggtgcca ctcacttttt tcaagttgat
      121 aacggactag ccttatttta acttgctatt tctagctcta aaacagagag ataacccaca
      181 agaattgagt taagcccatt cttcggtaca ggttatgttt tttggcaaca gcaactattc
      241 aaatcaactg aaagtttttt agaattcaac aacttccaat actcaagtgt ccaagctagc
      301 aaactgttta ataatcgtga cccaacattc ctttcatgcg caccctcagc gtcacataaa
      361 tcaacacttt acaacaccta ttcaaagctt ctatatgcct tcgtcattta aatgtctaac
      421 atttcaaaaa agtgtttgct aatggtaaat tcttgagttt tcttggaagc acatatataa
      481 cgagtgtgac tgttctacgc tccttgctga ctagtcatca gtctacgcat gccaactacc
      541 accaacataa gcaaaaggta cccggggtac cgagctcgaa ttcgtaatca tgtcatagct
      601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      901 atccacagaa tcag
//
