LOCUS       sequence-141049-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combined sgRNA/Cas9 vector with KanMX marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     sgRNA/Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMZ376
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(53..72)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 actcacgttt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
       61 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
      121 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      181 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      241 atccgtactc ctgatgatgc atggttactc accactgcga tccccggcaa aacagcattc
      301 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
      361 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
      421 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
      481 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
      541 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
      601 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
      661 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
      721 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
      781 gatgagtt
//
