LOCUS       sequence-141058-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT DDX3X in mammalian cells under blasticidin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: pMXs-DDX3X
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(558..577)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc
       61 cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca
      121 ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc
      181 tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg
      241 atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct
      301 gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga
      361 ttcttcttga gacaaaggaa ccatggttgt ggccatatta tcatcgtgtt tttcaaagga
      421 aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac taaacacatg
      481 taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc ttctgggcat
      541 ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc acaactatcc
      601 aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat acacgtggct
      661 tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta
      721 cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg acattcaaca
      781 gaccttgcat tccttttggc gagaggggaa agacccctag gaatgctcgt caa
//
