LOCUS       sequence-141069-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of 
            p-azido-l-phenylalanine, into proteins in E coli in response to the 
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAzFRS.1.t1
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcatcccca taatccttgt tagattatca attttaaaaa actaacagtt gtcagcctgt
       61 cccgctttaa tatcatacgc cgttatacgt tgtttacgct ttgaggaatc ccatatggac
      121 gaatttgaaa tgataaagag aaacacatct gaaattatca gcgaggaaga gttaagagag
      181 gttttaaaaa aagatgaaaa atctgctctg ataggttttg aaccaagtgg taaaatacat
      241 ttagggcatt atctccaaat aaaaaagatg attgatttac aaaatgctgg atttgatata
      301 attatattgt tggctgattt acacgcctat ttaaaccaga aaggagagtt ggatgagatt
      361 agaaaaatag gagattataa caaaaaagtt tttgaagcaa tggggttaaa ggcaaaatat
      421 gtttatggaa gtgaattcca gcttgataag gattatacac tgaatgtcta tagattggct
      481 ttaaaaacta ccttaaaaag agcaagaagg agtatggaac ttatagcaag agaggatgaa
      541 aatccaaagg ttgctgaagt tatctatcca ataatgcagg ttaatgttat gcattatgat
      601 ggcgttgatg tttatgttgg agggatggag cagagaaaaa tacacatgtt agcaagggag
      661 cttttaccaa aaaaggttgt ttgtattcac aaccctgtct taacgggttt ggatggagaa
      721 ggaaagatga gttcttcaaa agggaatttt atagctgttg atgactctcc agaagagatt
      781 agggctaaga taaagaaagc atactgccca gctggagttg ttgaaggaaa tccaataatg
      841 gagatagcta aatacttcct tgaatatcc
//
