LOCUS       sequence-141070-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C-terminal fusion of mRuby3 to CX43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pKanCMV-CX4.3-7aa-mRuby3
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      200..209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcaggccgga agcaccatct ccaactcgca cgcccagccg ttcgatttcc ccgacgacaa
       61 ccagaatgcc aaaaaagttg ctgctggaca tgaactccag ccattagcca tcgtggacca
      121 acgaccttcc agcagagcca gcagccgcgc cagcagcagg cctcggcctg atgacctgga
      181 gattgcggat ccaccggtcg ccaccatggt gtctaagggc gaagagctga tcaaggaaaa
      241 tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca aatgcacagg
      301 tgaaggagaa ggcagaccgt acgagggagt gcaaaccatg aggatcaaag tcatcgaggg
      361 aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg gcagccgtac
      421 ctttatcaag tacccggccg acatccctga tttctttaaa cagtcctttc ctgagggttt
      481 tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca cgcaggacac
      541 cagccttgag gatggcgagc tcgtctacaa cgtcaaggtc agaggggtaa actttccctc
      601 caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag agatgatgta
      661 tccagcagat ggtggtctga gaggatacac tgacatcgca ctgaaagttg atggtggtgg
      721 ccatctgcac tgcaacttcg tgacaactta caggtcaaaa aagaccgtcg ggaacatcaa
      781 gatgcccggt gtccatgccg ttgatcaccg cctggaaagg atcgaggaga gtgacaatga
      841 aacctacgta gtgcaaagag aagtggcagt tgccaaatac agcaacct
//
