LOCUS       sequence-141072-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of N-terminal fusion of mRuby3 to H2B..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pKanCMV-mRuby3-10aa-H2B
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    502..623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(539..558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     593..612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag
       61 aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac
      121 aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg
      181 aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat
      241 tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg
      301 cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc
      361 agcgctaagt aaggatccac cggatctaga taactgatca taatcagcca taccacattt
      421 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      481 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      541 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      601 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
      661 cgcgttaaat ttttgttaaa tcag
//
