LOCUS       sequence-141073-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of N-terminal fusion of mClover3 to H2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pKanCMV-mClover3-10aa-H2B
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..410
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     polyA_signal    540..661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(577..596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     631..650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(805..826)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agtccggact cagatctgga ggctccggag gcccagagcc agcgaagtct gctcccgccc
       61 cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca
      121 agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc
      181 ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt
      241 tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca
      301 cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg
      361 ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaagtaa ggatccaccg
      421 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      481 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      661 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
      721 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
      781 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
      841 gactccaacg tcaaagggcg a
//
