LOCUS       sequence-141119-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, 
            Mercader N, Concordet JP, Del Bene F
  TITLE     2C-Cas9: a versatile tool for clonal analysis of gene function.
  JOURNAL   Genome Res. 2016 Mar 8.
  PUBMED    26957310
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2016 Mar 8."
COMMENT     Sequence Label: pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    608..702
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
       61 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
      121 ttatttacag tcaaaaagta cttatttttt ggagatcact tcattctatt ttcccttgct
      181 attaccaaac caattgaatt gcgctgatgc ccagtttaat ttaaatagat cccatgggtc
      241 gactacgctt acagctccaa aactctacgg taaacctaca taaactgctg gttttcaaat
      301 tttaaagaat ttaagggttt acaggtttac tactacacag tgatttactg acacatgtaa
      361 gtgtaaatga gttgaataag taagtaagcc atataccaca catgaaacac atacccagaa
      421 gtcactggta tatatagccg tcctccagac tcccagctcg agagaccgag agagggtctc
      481 agtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
      541 cttgaaaaag tggcaccgag tcggtgcttt ttttggtcga cggatccaag cttgcatgcc
      601 tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      661 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagcgagcg
      721 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
//
