LOCUS       sequence-141122-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-J23100
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(49..106)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    179..198
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      201..244
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(248..266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(494..513)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt
       61 taaagaaaaa gggcagggtg gtgacacctt gccctttttt gccggactgc agctaatgag
      121 acggcatggg tctctgtcct ttctcctctt tgtactgaga cctatgcgtc tctagattct
      181 agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag ctaaggattt tttttatctg
      241 aaattctgcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      301 tagacgtcag gtggcactcg agttgatcgg gcacgtaaga ggttccaact ttcaccataa
      361 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
      421 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      481 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      541 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      601 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa
      661 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
      721 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
      781 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      841 tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca gttttgattt
      901 aaacgtggcc aatatggaca acttcttcgc cc
//
