LOCUS       sequence-141131-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash4
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             740..757
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 tctccggcga gctctcgaga attctcacgc gtctgcagga tatcaagctt gcgttaccgg
       61 atccatgggt gaaaaaccag ggaccagggt cttcaagaag tcgagcccta actgcaagct
      121 caccgtgtac ttgggcaagc gtgactttgt ggatcacttg gacaaagtgg atcctgtcga
      181 tggtgtggtg cttgtggatc ctgactactt gaaggaccgg aaagtgtttg tgaccctcac
      241 ctgtgccttc cgctatggcc gagaagacct ggatgtactg ggcctgtctt tccgcaaaga
      301 tctgttcatc gccacctacc aggccttccc ccccatgccc aacccacctc ggccccccac
      361 ccgcctacag gaccgactgc tgaagaagtt gggccagcat gcccacccct tttttttcac
      421 aataccccag aatttgcctt gctccgtcac actgcagcca ggaccggagg acacagggaa
      481 ggcctgtgga gtagactttg agattcgagc cttctgtgcc aaatctatag aagaaaaaag
      541 ccacaaaagg aactccgtgc ggcttatcat cagaaaggta cagtttgctc ctgagacacc
      601 cggcccccag ccatcagctg aaaccacacg ccacttcctc atgtctgacc ggaggtccct
      661 gcacctagag gcttccctgg acaaagagct gtactaccat ggggaacccc tcaatgtcaa
      721 cgtccacgtc accaacaatt gttgtcctgg ttgttgttct gccaagaccg tcaagaagat
      781 cagagtgtct gtgagacagt atgccgacat ttgcctcttc agcaccgcgc agtacaagtg
      841 tcctgtggct cagcttgaac aagatgacca ggtgtctccc agttccacat tctgca
//
